The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin
The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin
The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin by David Kateete
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Author: David Kateete
Number of Pages: 92 pages
Published Date: 19 Mar 2010
Publisher: LAP Lambert Academic Publishing
Publication Country: Saarbrucken, Germany
Language: English
Format: eBook
ISBN: 9783838351407
Download Link: The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin
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T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei.
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The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin by David Kateete
Author: David Kateete
Number of Pages: 92 pages
Published Date: 19 Mar 2010
Publisher: LAP Lambert Academic Publishing
Publication Country: Saarbrucken, Germany
Language: English
Format: eBook
ISBN: 9783838351407
Download Link: The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin
---------------------------------------------------------------
T. b. brucei alpha and beta tubulin genes were expressed in E. coli using the pTrP2 expression plasmid and 3- -Indoleacrylic acid as an inducer. The expressed tubulins were analysed by SDS-PAGE and confirmed by Western blotting, in which they had an identical apparent molecular mass of 50kDa. The expressed tubulins were individually dissolved in 8M Urea and diluted 20-fold, after which they were concentrated and extensively dialyzed against MES buffer. Following dialysis, the average yield for both alpha and beta tubulin was 0.7g per liter of culture. Polyclonal antibodies were produced in each of the 56 rabbits immunized with three doses of the concentrated tubulins at either 150 g or 450 g per immunization per animal. The effect of these antibodies on T. b. brucei growth was studied by culturing T. b. brucei bloodstream form in up to 25% of the rabbit-raised antisera. The antisera from the other rabbits did not inhibit T. b. brucei growth in culture. These results show that antibodies to bacterially expressed trypanosome tubulin may not be cytotoxic to T. b. brucei.
Read online The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin Buy and read online The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin Download The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent Download to iPad/iPhone/iOS, B&N nook The Trypanocidal Effects of Antibodies to T.Brucei-Recombinant Tubulin
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